Hurnik D, Hanna P E, Dohoo I R
Atlantic Veterinary College, University of Prince Edward Island, Charlottetown.
Can J Vet Res. 1993 Jan;57(1):37-41.
A rapid gross visual appraisal of enzootic pneumonia lesions was made of 87 lungs at a local abattoir and the lungs were then set aside and examined in more detail. A sample of pulmonary tissue was taken from each lung and submitted for bacterial and histological examination. The principal investigator who had performed the gross and detailed lung scores was then used to assess the agreement of two inspectors who were scoring lung lesions in the abattoir. The Pearson's correlation coefficient between grossly scored lungs and scores derived from a detailed examination was 0.94. Using histological examination as the gold standard, rapid gross examination had a sensitivity of 76% and a specificity of 71%. Using bacterial recovery as a gold standard yielded a sensitivity of 77% and a specificity of 51%. The sensitivity and specificity of inspectors 1 and 2 compared to the principal investigator were: sensitivity = 97.5% and specificity = 97.4% for inspector 1, and 97% and 98% for inspector 2. The kappa values for both of the inspectors compared to the principal investigator were 0.95 suggesting that designated lay inspectors consistently agreed with the principal investigator.
在当地一家屠宰场对87个肺脏进行了猪地方性肺炎病变的快速大体视觉评估，然后将这些肺脏留存下来做更详细的检查。从每个肺脏中采集一份肺组织样本，送去做细菌学和组织学检查。由进行了大体和详细肺脏评分的主要研究者来评估两名在屠宰场对肺脏病变进行评分的检查员之间的一致性。大体评分的肺脏与详细检查得出的评分之间的皮尔逊相关系数为0.94。以组织学检查作为金标准，快速大体检查的敏感性为76%，特异性为71%。以细菌培养结果作为金标准时，敏感性为77%，特异性为51%。与主要研究者相比，检查员1和检查员2的敏感性和特异性分别为：检查员1的敏感性 = 97.5%，特异性 = 97.4%；检查员2的敏感性 = 97%，特异性 = 98%。与主要研究者相比，两名检查员的kappa值均为0.95，这表明指定的非专业检查员与主要研究者的意见始终一致。
