Bunyaratvej A, Butthep P, Bunyaratvej P
Department of Pathology and Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Cytometry. 1993;14(1):81-5. doi: 10.1002/cyto.990140114.
Blood samples from malaria-infected patients and from in vitro culture were analyzed using the H*1 hematology analyzer. An attempt to find a hematologic parameter for detecting the malaria infection and to characterize the pathophysiological changes of red cells was made. The study included 18 malaria-infected patients (10 with Plasmodium falciparum and 8 with Plasmodium vivax) and 52 normal, healthy volunteers. Increased young large lymphocyte or large unstained cell count (LUC over 3%) in the peripheral blood of malaria-infected patients was evidence for malaria infection. Increased population dispersions of red cell volume (red cell distribution width: RDW) and intraerythrocytic hemoglobin concentration (hemoglobin distribution width: HDW) were demonstrated both in clinical samples and cultured blood. The increased RDW correlated with an increased percentage of macrocytes (r = 0.64, P = 0.004). Comparison of HDW and percentage of hypochromic red cells between the clinical specimen and the cultured blood supports the finding that changes in red cell hemoglobin concentration were mainly due to the response of the patient to malaria infection and partly due to the effect of malaria parasites on the red cells.
使用H*1血液分析仪对疟疾感染患者的血样和体外培养样本进行了分析。旨在寻找用于检测疟疾感染的血液学参数并描述红细胞的病理生理变化。该研究纳入了18例疟疾感染患者（10例感染恶性疟原虫，8例感染间日疟原虫）和52名正常健康志愿者。疟疾感染患者外周血中年轻大淋巴细胞或大未染色细胞计数增加（LUC超过3%）是疟疾感染的证据。临床样本和培养血液中均显示红细胞体积的群体离散度增加（红细胞分布宽度：RDW）和红细胞内血红蛋白浓度增加（血红蛋白分布宽度：HDW）。RDW增加与大红细胞百分比增加相关（r = 0.64，P = 0.004）。临床样本与培养血液中HDW和低色素红细胞百分比的比较支持以下发现：红细胞血红蛋白浓度的变化主要是由于患者对疟疾感染的反应，部分是由于疟原虫对红细胞的影响。
