Char S, Cevallos A M, Yamson P, Sullivan P B, Neale G, Farthing M J
Department of Gastroenterology, St Bartholomew's Hospital, London.
Gut. 1993 Jan;34(1):38-40. doi: 10.1136/gut.34.1.38.
The serum antibody response in Gambian children with persistent diarrhoea and giardiasis has been studied. Total serum IgG, IgA, and IgM concentrations were increased in these patients as compared with controls from the same area. Determination of the concentrations of Giardia specific antibodies by enzyme linked immuno adsorbent assay (ELISA), however, revealed that only IgM was raised while those of IgA and IgG were similar to the controls. Analysis of the antigenic determinants of the IgG and IgA responses by immunoblotting showed that patients with chronic infection unlike those who clear the infection have no IgA response to a 57 kDa Giardia heat shock antigen. The association of high concentrations of Giardia specific IgM, low concentrations of Giardia specific IgA and IgG and inability to clear the infection suggests that the switch from an IgM to an IgG or IgA response is inefficient.
对冈比亚患有持续性腹泻和贾第虫病的儿童的血清抗体反应进行了研究。与来自同一地区的对照组相比，这些患者的血清总IgG、IgA和IgM浓度有所升高。然而，通过酶联免疫吸附测定（ELISA）测定贾第虫特异性抗体的浓度发现，只有IgM升高，而IgA和IgG的浓度与对照组相似。通过免疫印迹分析IgG和IgA反应的抗原决定簇表明，与清除感染的患者不同，慢性感染患者对57 kDa贾第虫热休克抗原没有IgA反应。高浓度的贾第虫特异性IgM、低浓度的贾第虫特异性IgA和IgG以及无法清除感染之间的关联表明，从IgM反应向IgG或IgA反应的转换效率低下。
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