Department of Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Parasitol Res. 2012 Aug;111(2):571-5. doi: 10.1007/s00436-012-2869-y. Epub 2012 Mar 9.
Giardiasis is a gastrointestinal infection of wide distribution that is more prevalent in childhood. Easy and rapid diagnosis of giardiasis is essential for reduction of this infection. This cross-sectional study included 62 children in which collection of saliva, stool and serum samples was performed. An enzyme-linked immunosorbent assay (ELISA) technique was evaluated to detect IgA and IgG responses in both saliva and serum samples. Twenty-two children were positive for Giardia duodenalis infection by direct examination of faecal specimens, 20 non-infected and 20 infected with other parasites. Salivary and serum IgA and IgG responses against G. duodenalis infection were significantly higher in Giardia parasitized than non-Giardia parasitized children (p < 0.001). This concludes that specific salivary IgA may serve as a diagnostic tool and specific salivary IgG as a screening tool in monitoring the exposure of various populations to Giardia duodenalis. The advantage of salivary assays over serum immunoglobulin assay is being easy and non-invasive in sampling technique which is important especially for young children.
贾第虫病是一种分布广泛的胃肠道感染，在儿童中更为常见。贾第虫病的简便快速诊断对于减少这种感染至关重要。本横断面研究纳入了 62 名儿童，采集了唾液、粪便和血清样本。评估了酶联免疫吸附试验（ELISA）技术，以检测唾液和血清样本中的 IgA 和 IgG 反应。22 名儿童的粪便直接检查呈十二指肠贾第鞭毛虫感染阳性，20 名未感染和 20 名感染其他寄生虫。与非贾第鞭毛虫感染儿童相比，贾第虫感染的儿童唾液和血清 IgA 和 IgG 反应显著升高（p<0.001）。这表明特异性唾液 IgA 可作为诊断工具，特异性唾液 IgG 可作为监测各种人群接触十二指肠贾第鞭毛虫的筛查工具。唾液检测相对于血清免疫球蛋白检测的优势在于采样技术简便无创，这对于幼儿尤为重要。
