通过特异性IgM抗体酶联免疫吸附测定诊断贾第虫病。

Diagnosis of giardiasis by specific IgM antibody enzyme-linked immunosorbent assay.

作者信息

Goka A K, Rolston D D, Mathan V I, Farthing M J

出版信息

Lancet. 1986 Jul 26;2(8500):184-6. doi: 10.1016/s0140-6736(86)92487-6.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was used to detect serum IgM and IgG antibodies against Giardia in fifty-two patients with diarrhoea from the United Kingdom and south India. Serum anti-Giardia IgM responses occurred in patients with giardiasis; in two of three patients studied longitudinally IgM levels had fallen to normal 2-3 weeks after treatment. At serum dilutions of 1/200 to 1/400 there was almost complete separation of ELISA readings between patients with Giardia and Giardia-free controls; the sensitivity and specificity of the ELISA were both 96%. Serum anti-Giardia IgG was detected in patients and controls but was unable to distinguish current from previous infection.

摘要

采用酶联免疫吸附试验（ELISA）检测了来自英国和印度南部的52例腹泻患者血清中抗贾第虫的IgM和IgG抗体。贾第虫病患者出现血清抗贾第虫IgM反应；在纵向研究的3例患者中，有2例在治疗后2 - 3周IgM水平已降至正常。在血清稀释度为1/200至1/400时，贾第虫感染患者与未感染贾第虫的对照之间的ELISA读数几乎完全分离；ELISA的敏感性和特异性均为96%。在患者和对照中均检测到血清抗贾第虫IgG，但无法区分当前感染与既往感染。