Trask B, Fertitta A, Christensen M, Youngblom J, Bergmann A, Copeland A, de Jong P, Mohrenweiser H, Olsen A, Carrano A
Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94550.
Genomics. 1993 Jan;15(1):133-45. doi: 10.1006/geno.1993.1021.
We report here the band location of 540 cosmids mapped to chromosome 19. The cosmids were mapped by fluorescence in situ hybridization (FISH) relative to chromosomal bands produced by DAPI/actinomycin staining. The cosmids are distributed throughout the chromosome, with a sampling bias for the q-arm. A detailed analysis of the distribution of three different subtelomeric and 22 pericentromeric chromosome 19 cosmids on other chromosomes is also reported. Colony hybridization identified 142 cosmids that contain sequences representing genes or DNA markers that map to chromosome 19. FISH mapping of these cosmids sublocalizes a total of 70 genes and DNA markers on chromosome 19, revises the previously published map assignments of 2 genes, and narrows the location of over 20 markers.
我们在此报告定位到19号染色体的540个黏粒的条带位置。这些黏粒通过荧光原位杂交（FISH）相对于由DAPI/放线菌素染色产生的染色体条带进行定位。黏粒分布于整个染色体，在q臂存在抽样偏差。本文还报告了对19号染色体上三种不同的亚端粒和22个着丝粒周围黏粒在其他染色体上分布情况的详细分析。菌落杂交鉴定出142个黏粒，其包含定位到19号染色体的代表基因或DNA标记的序列。这些黏粒的FISH定位将总共70个基因和DNA标记亚定位到19号染色体上，修订了之前发表的2个基因的图谱定位，并缩小了20多个标记的位置范围。
