Opdahl H
Institute of Physiology, University of Oslo, Norway.
Inflammation. 1993 Feb;17(1):57-75. doi: 10.1007/BF00916392.
Polymorphonuclear neutrophil granulocytes (PMN) may contribute to the lung injury induced by nonpulmonary infections with gram-negative bacteria. The direct effect of E. coli lipopolysaccharide (LPS) on isolated human PMN or mixed leukocytes (ML), as well as the priming effect of preincubating cells with LPS, was examined in assays measuring the maximal rate of oxygen consumption (OC), cell chemiluminescence (CHML), and aggregation (AGG). LPS, 1-10 micrograms/ml, caused no acute response in PMN or ML suspended in Fisher's-HEPES medium with BSA (FHA), but increased both CHML and AGG of cells suspended in autologous plasma. Preincubation in FHA with LPS, 1 microgram/ml, for more than 15 min increased the OC of PMN activated with zymosan-activated plasma (ZAP) or n-formyl-methionyl-leu-cyl-phenylalanine (FMLP) by more than 100%. A similar increase in the CHML of such cells was seen after FMLP, but not after ZAP. ZAP, however, primed the CHML response of the cells to subsequent activation with FMLP more than did preincubation with LPS. Previous exposure to both agents had an additive effect. Preincubation of PMN with LPS decreased the time interval from addition of phorbol myristate acetate (PMA) to peak OC response, but less so than previous activation with FMLP. Neither agent affected the maximal rate of OC after addition of PMA. LPS also increased the PMN aggregation induced by ZAP and FMLP, but not by PMA. Cells preincubated with LPS, 0.01 microgram/ml, increased their CHML in response to FMLP if suspended in Krebs-Ringer balanced salt solution, but not if suspended in FHA. Such preincubation had no effect on OC of similarly activated cells in any of the media.
多形核中性粒细胞（PMN）可能参与革兰氏阴性菌非肺部感染所致的肺损伤。在测量最大耗氧率（OC）、细胞化学发光（CHML）和聚集（AGG）的实验中，研究了大肠杆菌脂多糖（LPS）对分离的人PMN或混合白细胞（ML）的直接作用，以及细胞与LPS预孵育的引发作用。1 - 10微克/毫升的LPS对悬浮于含牛血清白蛋白（BSA）的Fisher's - HEPES培养基（FHA）中的PMN或ML无急性反应，但可增加悬浮于自体血浆中的细胞的CHML和AGG。在FHA中用1微克/毫升LPS预孵育超过15分钟，可使经酵母聚糖激活血浆（ZAP）或N - 甲酰甲硫氨酰 - 亮氨酰 - 苯丙氨酸（FMLP）激活的PMN的OC增加100%以上。FMLP刺激后，此类细胞的CHML也有类似增加，但ZAP刺激后无此现象。然而，ZAP比LPS预孵育更能引发细胞对后续FMLP激活的CHML反应。先前暴露于这两种物质有相加作用。PMN与LPS预孵育可缩短从加入佛波酯肉豆蔻酸酯乙酸盐（PMA）到OC反应峰值的时间间隔，但比先前用FMLP激活的作用小。两种物质均不影响加入PMA后的最大OC率。LPS还可增加ZAP和FMLP诱导的PMN聚集，但不增加PMA诱导的聚集。用0.01微克/毫升LPS预孵育的细胞，如果悬浮于Krebs - Ringer平衡盐溶液中，对FMLP刺激的CHML增加，但悬浮于FHA中则无此现象。这种预孵育对任何一种培养基中类似激活细胞的OC均无影响。
