Hale Y M, Melton M E, Lewis J S, Willis D E
Office of Laboratory Services, State of Florida, Health and Rehabilitative Services, Jacksonville 32202.
J Clin Microbiol. 1993 Feb;31(2):451-3. doi: 10.1128/jcm.31.2.451-453.1993.
The Gen-Probe PACE 2 DNA probe assay for Neisseria gonorrhoeae was compared with conventional culture techniques in three Florida public health laboratories with 436 patients (271 females and 165 males). The prevalence rates based on culture were 19.9, 55.8, and 33.5% for females, for males, and overall, respectively. Twenty-seven probe-positive specimens gave negative culture results. Twenty of these specimens were resolved as true positives after retesting with a probe competition assay. The resolved sensitivity, specificity, positive predictive value, and negative predictive value were 99.4, 99.6, 99.4, and 99.6%, respectively.
在佛罗里达州的三个公共卫生实验室中，将用于淋病奈瑟菌检测的基因探针PACE 2 DNA探针检测法与传统培养技术对436名患者（271名女性和165名男性）进行了比较。基于培养的患病率，女性为19.9%，男性为55.8%，总体为33.5%。27个探针阳性标本培养结果为阴性。其中20个标本在使用探针竞争检测法重新检测后被判定为真阳性。最终的灵敏度、特异性、阳性预测值和阴性预测值分别为99.4%、99.6%、99.4%和99.6%。
