Bourdoulous S, Durieu-Trautmann O, Strosberg A D, Couraud P O
Institut Cochin de Génétique Moléculaire, CNRS UPR 0415, Paris, France.
J Immunol. 1993 Feb 15;150(4):1486-95.
Bovine brain microvessel endothelial cells in primary culture retain a differentiated phenotype reminiscent of the in vivo blood-brain barrier endothelium. The IFN-gamma-induced surface expression of MHC class II molecules on those cells is stimulated by catecholamines through a cAMP-independent mechanism. We report that both the induction of MHC class II molecule expression by IFN-gamma and its potentiation by isoproterenol, a catecholamine analog, are preceded by increases of steady-state levels of the corresponding mRNA. Similar results were obtained for the regulation of invariant chain expression. In addition, isoproterenol alone is able to increase class I mRNA levels, but, in contrast to what is observed for class II expression, this effect appears to be mainly mediated by cAMP. These studies demonstrate that, in bovine brain microvessel endothelial cells, catecholamines up-regulate MHC class I and class II Ag, as well as invariant chain with different kinetics and through different mechanisms and suggest that these regulatory effects occur at the transcriptional level.
原代培养的牛脑微血管内皮细胞保留了一种分化表型，让人联想到体内血脑屏障内皮细胞。儿茶酚胺通过一种不依赖cAMP的机制刺激这些细胞上由干扰素-γ诱导的MHC II类分子的表面表达。我们报告，干扰素-γ诱导MHC II类分子表达以及儿茶酚胺类似物异丙肾上腺素对其的增强作用，均先于相应mRNA稳态水平的增加。对于恒定链表达的调节也获得了类似结果。此外，单独的异丙肾上腺素能够增加I类mRNA水平，但与II类表达所观察到的情况相反，这种效应似乎主要由cAMP介导。这些研究表明，在牛脑微血管内皮细胞中，儿茶酚胺以不同的动力学和通过不同的机制上调MHC I类和II类抗原以及恒定链，并提示这些调节作用发生在转录水平。
