Reis D D, Gazzinelli R T, Gazzinelli G, Colley D G
Department de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Brazil.
J Immunol. 1993 Feb 15;150(4):1611-8.
Immunization of rabbits with pools of immunoaffinity-purified anti-Trypanosoma cruzi epimastigote antibodies derived from patients with different clinical forms of Chagas' disease induces antiidiotypic sera that can distinguish between anti-epimastigote antibodies from patients with asymptomatic (indeterminate (IND)) or severe (cardiac (CARD)) Chagas' disease. These idiotypically different anti-EPI antibodies from patients with the different clinical forms do not differ in their anti-epimastigote activities or isotypes. Analysis of immunoaffinity purified antibodies from individual chagasic patients by specific competitive ELISA generally confirms that Id-specific rabbit antisera can differentiate the clinical forms of the source of the antibodies. Based on these data, immunoaffinity-purified antibodies from patients share many Id with those from IND patients, although antibodies from IND patients express much lower levels of the distinctive Id characteristic of CARD patients. Reduction and alkylation of antibodies from IND patients reduces somewhat, but does not abolish, the ability of their Id to be recognized idiotypically, and to effectively inhibit in competitive ELISA. In contrast, reduction and alkylation of antibodies from CARD patients almost completely eliminates the ability of their predominant Id to be either recognized by, or inhibit, the appropriate systems. These data imply that the expression of the major Id that define CARD patients by these serologic anti-Id systems is largely dependent on the tertiary conformation of the Ig molecule. This agrees with our earlier studies on the respective differential abilities of CARD vs IND Id to stimulate anti-Id T cells by direct stimulation vs processing and presentation mechanisms.
用从患有不同临床类型恰加斯病的患者中获得的免疫亲和纯化的抗克氏锥虫前鞭毛体抗体池免疫兔子，可诱导产生抗独特型血清，该血清能够区分无症状（不确定型（IND））或严重（心脏型（CARD））恰加斯病患者的抗前鞭毛体抗体。这些来自不同临床类型患者的独特型不同的抗前鞭毛体抗体在抗前鞭毛体活性或同种型方面并无差异。通过特异性竞争ELISA对个别恰加斯病患者的免疫亲和纯化抗体进行分析，总体上证实了独特型特异性兔抗血清能够区分抗体来源的临床类型。基于这些数据，来自患者的免疫亲和纯化抗体与来自IND患者的抗体有许多共同独特型，尽管来自IND患者的抗体表达的CARD患者特有的独特型水平要低得多。对来自IND患者的抗体进行还原和烷基化处理后，其独特型被独特型识别以及在竞争ELISA中有效抑制的能力有所降低，但并未消除。相比之下，对来自CARD患者的抗体进行还原和烷基化处理几乎完全消除了其主要独特型被相应系统识别或抑制的能力。这些数据表明，通过这些血清学抗独特型系统定义CARD患者的主要独特型的表达在很大程度上取决于Ig分子的三级构象。这与我们早期关于CARD与IND独特型通过直接刺激与加工呈递机制刺激抗独特型T细胞的各自不同能力的研究结果一致。
