Saroff H A
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Anal Biochem. 1993 Jan;208(1):88-95. doi: 10.1006/abio.1993.1012.
Linked ligand functions for analyzing experimental data evaluated to yield macroscopic apparent binding constants were well developed by Adair and by Wyman. In this paper, expressions for the linked function (derivative) delta ln(K)/delta ln(c) are applied to both macroscopic association constants and individual-site association constants derived from individual-site binding data. A detailed treatment of a simple interacting system is presented. We demonstrate that data from individual-site isotherms can be interpreted only after explicit assumptions specifying the nature of the ligand-dependent interactions are made.
用于分析实验数据以得出宏观表观结合常数的连锁配体函数由阿代尔和怀曼充分发展起来。在本文中，连锁函数（导数）δln(K)/δln(c)的表达式被应用于宏观缔合常数以及从单位点结合数据推导得到的单位点缔合常数。本文给出了一个简单相互作用系统的详细处理。我们证明，只有在做出明确假设以指定配体依赖性相互作用的性质之后，单位点等温线的数据才能得到解释。
