Czerlinski G H
Northwestern University, Chicago, IL 60611.
Physiol Chem Phys Med NMR. 1989;21(1):107-12.
Differential analysis of allosteric systems focuses on the ratio of consecutive binding constants obtained for multiple binding as originally used by Adair. If this ratio of experimental constants is smaller than the ratio of statistical constants for multiple binding, cooperativity among subunits is indicated. The conditions are evaluated with the aid of the model of Monod et al and compared with available data on calmodulin. Calmodulin shows cooperativity among some subunits, not among others, depending strongly upon experimental details. Values of Ln (the interconversion constant of fully occupied sites) can be estimated for c much less than 1 (and Ln-1 much greater than 1).
变构系统的差异分析集中于如阿代尔最初所使用的，针对多重结合所获得的连续结合常数的比率。如果实验常数的这一比率小于多重结合的统计常数的比率，则表明亚基之间存在协同性。借助莫诺等人的模型对这些条件进行评估，并与钙调蛋白的现有数据进行比较。钙调蛋白在一些亚基之间显示出协同性，而在其他亚基之间则不然，这在很大程度上取决于实验细节。对于远小于1的c（且Ln - 1远大于1），可以估算出Ln（完全占据位点的相互转换常数）的值。
