Kubota Y, Jones E G
Laboratory for Neural Systems, RIKEN, Saitama, Japan.
Brain Res. 1993 Jan 15;600(2):339-44. doi: 10.1016/0006-8993(93)91394-8.
In the rat piriform cortex, double-fluorescence immunocytochemistry was used to study the coexistence of two calcium binding proteins, parvalbumin and calbindinD28k, and GABA. Layer I cells were immunoreactive only for GABA. In layer II a small number of cells were immunoreactive for all three substances. A few other cells contained only parvalbumin and GABA immunoreactivity. In layer III a subpopulation of calbindinD28k immunoreactive cells (about 60%) were also immunoreactive for both parvalbumin and GABA. The remainder showed only calbindinD28k and GABA immunoreactivity. Many immunoreactive terminals, which surrounded non-immunoreactive cell somata to form basket endings in layers II and III, were immunoreactive for all three substances. Their major source may be the non-pyramidal neurons in layer III, which contained the three chemical substances.
在大鼠梨状皮质中，采用双荧光免疫细胞化学法研究两种钙结合蛋白小清蛋白和钙结合蛋白D28k与γ-氨基丁酸（GABA）的共存情况。I层细胞仅对GABA呈免疫反应性。在II层，少数细胞对这三种物质均呈免疫反应性。其他一些细胞仅含有小清蛋白和GABA免疫反应性。在III层，一部分钙结合蛋白D28k免疫反应性细胞（约60%）对小清蛋白和GABA也呈免疫反应性。其余细胞仅显示钙结合蛋白D28k和GABA免疫反应性。许多免疫反应性终末在II层和III层围绕非免疫反应性细胞体形成篮状终末，对这三种物质均呈免疫反应性。它们的主要来源可能是III层中含有这三种化学物质的非锥体神经元。
