Sugie S, Yoshimi N, Okumara A, Tanaka T, Mori H
Department of Pathology, Gifu University School of Medicine, Japan.
Carcinogenesis. 1993 Feb;14(2):281-3. doi: 10.1093/carcin/14.2.281.
The effects of benzyl isothiocyanate (BITC) and benzyl thiocyanate (BTC) on two types of DNA synthesis were examined in hepatocyte primary cultures (HPC). Male F344 rats were fed BITC- or BTC-containing diets at a concentration of 400 p.p.m. Using hepatocytes isolated from these rats, DNA repair was measured by unscheduled DNA synthesis (UDS) for some genotoxic carcinogens, e.g. 2-acetylaminofluorene (AAF), methylazoxymethanol (MAM) acetate, 9,10-dimethyl-1,2-benzanthracene (DMBA) and diethylnitrosamine (DEN), and compared with that in the hepatocytes from rats without BITC or BTC treatment. Replicative DNA synthesis (RDS) was also evaluated in the hepatocytes of rats with or without thiocyanate treatment. Both BITC and BTC reduced UDS elicited by these carcinogens. The level of RDS in the hepatocytes of rats exposed to BITC or BTC was markedly lower than in the cells of rats without BITC or BTC exposure. These results indicate that in vivo exposure to BITC and BTC suppressed carcinogen-induced genotoxicity and cell proliferative activity and suggest that this assay may prove useful in detecting chemopreventive agents for cancer and in investigating the properties of carcinogenesis modifiers.
在原代肝细胞培养（HPC）中检测了异硫氰酸苄酯（BITC）和硫氰酸苄酯（BTC）对两种DNA合成类型的影响。给雄性F344大鼠喂食浓度为400 ppm的含BITC或BTC的饲料。使用从这些大鼠分离的肝细胞，通过非程序性DNA合成（UDS）测量某些遗传毒性致癌物（如2-乙酰氨基芴（AAF）、乙酸甲基偶氮甲醇（MAM）、9,10-二甲基-1,2-苯并蒽（DMBA）和二乙基亚硝胺（DEN））的DNA修复情况，并与未用BITC或BTC处理的大鼠的肝细胞中的DNA修复情况进行比较。还评估了有或没有硫氰酸盐处理的大鼠肝细胞中的复制性DNA合成（RDS）。BITC和BTC均降低了这些致癌物引发的UDS。暴露于BITC或BTC的大鼠肝细胞中的RDS水平明显低于未暴露于BITC或BTC的大鼠细胞中的RDS水平。这些结果表明，体内暴露于BITC和BTC可抑制致癌物诱导的遗传毒性和细胞增殖活性，并表明该检测方法可能在检测癌症化学预防剂和研究致癌作用修饰剂的特性方面证明有用。
