人淋巴细胞对分化型肠道肿瘤细胞系的细胞毒活性。
Cytotoxic activity of human lymphocytes against differentiated intestinal tumour cell lines.
作者信息
Anelli R, Placido R, Sambuy Y, Bach S, Di Massimo A, Colizzi V
机构信息
Department of Biology, University of Rome Tor Vergata, Italy.
出版信息
Immunology. 1993 Jan;78(1):166-9.
The natural killer (NK) and lymphokine-activated killer (LAK) cytotoxic activity of human peripheral blood lymphocytes (PBL) against various human tumour cell lines from intestinal origin (WIDR, HT29, Caco-2) has been investigated. The differentiated Caco-2 cells were then used as a model to investigate the cytotoxic activity against enterocyte-like target cells. Caco-2 were seeded on polycarbonate filters and maintained in culture for at least 15 days to allow the differentiation and formation of tight junctions. The integrity of tight junctions was assayed by measuring [3H]mannitol flux from apical to basolateral compartment. Cytotoxic analysis showed that both differentiated and undifferentiated Caco-2 cells were similarly susceptible to NK and LAK activity. The capacity of cytotoxic lymphocytes to kill enterocyte-like cells with intact junctional complex may suggest a direct role of cytotoxic lymphocytes in causing intestinal lesions under inflammatory conditions.
研究了人外周血淋巴细胞（PBL）对多种肠道来源的人肿瘤细胞系（WIDR、HT29、Caco-2）的自然杀伤（NK）和淋巴因子激活的杀伤（LAK）细胞毒性活性。然后将分化的Caco-2细胞用作模型，研究对肠上皮样靶细胞的细胞毒性活性。将Caco-2接种于聚碳酸酯滤膜上，在培养中维持至少15天，以使其分化并形成紧密连接。通过测量从顶端隔室到基底外侧隔室的[3H]甘露醇通量来测定紧密连接的完整性。细胞毒性分析表明，分化和未分化的Caco-2细胞对NK和LAK活性的敏感性相似。具有完整连接复合体的细胞毒性淋巴细胞杀伤肠上皮样细胞的能力可能表明细胞毒性淋巴细胞在炎症条件下引起肠道病变中起直接作用。
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