Cerf-Bensussan N, Guy-Grand D, Griscelli C
Gut. 1985 Jan;26(1):81-8. doi: 10.1136/gut.26.1.81.
A method using a mechanical procedure for isolation of lymphocytes from the epithelium of human intestinal mucosa allows the study of some of their characteristics and functions. Most of the isolated cells are of the T lineage (E+ and T3+) and express the phenotype associated with cytotoxic-suppressor T cells (T8). A large number contain intracytoplasmic granules. Granules are stained with alcian blue (pH 2.2), are metachromatic with Toluidine blue (pH 4) and some are shown to incorporate 35sulphate, suggesting that they contain sulphated mucopolysaccharides. As these cells are similar in many respects to the large granular lymphocytes that mediate natural killer activity in the peripheral blood, their natural cytotoxicity was tested against K 562 target cells. No activity was detected among the human intraepithelial lymphocytes and treatments with known potentiators of natural killer activity, ie, interferon or PHA-depleted conditioned medium containing Il-2, failed to reveal any cytotoxic activity.
一种使用机械方法从人肠黏膜上皮中分离淋巴细胞的方法，能够用于研究这些细胞的某些特性和功能。大多数分离出的细胞属于T细胞谱系（E+和T3+），并表达与细胞毒性抑制性T细胞（T8）相关的表型。大量细胞含有胞质颗粒。颗粒用阿尔辛蓝（pH 2.2）染色，用甲苯胺蓝（pH 4）呈异染性，并且一些颗粒显示掺入了35硫酸盐，这表明它们含有硫酸化的粘多糖。由于这些细胞在许多方面与在外周血中介导自然杀伤活性的大颗粒淋巴细胞相似，因此针对K 562靶细胞测试了它们的自然细胞毒性。在人上皮内淋巴细胞中未检测到活性，并且用已知的自然杀伤活性增强剂（即干扰素或含IL-2的PHA耗尽的条件培养基）处理后，也未发现任何细胞毒性活性。
