Mavaddat N, Robinson B W, Rose A H, Manning L S, Garlepp M J
Department of Medicine, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands.
Immunol Cell Biol. 1993 Feb;71 ( Pt 1):27-37. doi: 10.1038/icb.1993.3.
gamma delta T cells are capable of mediating non-major histocompatibility complex (MHC) restricted lysis of a variety of tumour cell lines. The mechanism of this lysis and its significance in tumour immunity are not clear. We have used a panel of five malignant mesothelioma (MM) cell lines, as well as standard tumour targets K562 and Daudi, to investigate some of the factors which could be involved in non-MHC restricted cytotoxicity mediated by gamma delta T cells. Individual MM cell lines, representing a panel of lines derived from a single cell type, varied in their susceptibility to lysis by gamma delta T cell clones. Individual gamma delta T cell clones also showed unique cytotoxic profiles, and differed in their cytotoxic potential. T cell receptor (TCR) V gamma gene usage correlated with the ability of clones to lyse Daudi or K562; clones lysing Daudi expressing V gamma 9 and clones lysing K562 expressing V gamma I subgroup genes. No strict correlation between V gamma and V delta gene usage and MM reactivity was, however, demonstrable. There was also no correlation between gamma delta T cell lysis of MM cell lines and the capacity of gamma delta T cells to produce interferon-gamma, tumour necrosis factor-alpha, interleukin-2 or interleukin-4, nor with their expression of CD8.
γδ T细胞能够介导对多种肿瘤细胞系的非主要组织相容性复合体（MHC）限制性裂解。这种裂解的机制及其在肿瘤免疫中的意义尚不清楚。我们使用了一组五种恶性间皮瘤（MM）细胞系，以及标准肿瘤靶细胞K562和Daudi，来研究一些可能参与γδ T细胞介导的非MHC限制性细胞毒性的因素。代表源自单一细胞类型的一组细胞系的各个MM细胞系，对γδ T细胞克隆裂解的敏感性各不相同。各个γδ T细胞克隆也表现出独特的细胞毒性谱，并且在细胞毒性潜力方面存在差异。T细胞受体（TCR）Vγ基因的使用与克隆裂解Daudi或K562的能力相关；裂解Daudi的克隆表达Vγ9，裂解K562的克隆表达VγI亚组基因。然而，Vγ和Vδ基因的使用与MM反应性之间没有明显的相关性。MM细胞系的γδ T细胞裂解与γδ T细胞产生干扰素-γ、肿瘤坏死因子-α白细胞介素-2或白细胞介素-4的能力之间也没有相关性，与它们的CD8表达也没有相关性。
