Wataha J C, Hanks C T, Craig R G
University of Michigan School of Dentistry, Ann Arbor 48109-1078.
J Biomed Mater Res. 1993 Feb;27(2):227-32. doi: 10.1002/jbm.820270212.
Atomic absorption spectroscopy was used to assess uptake of Ag+1, Au+4, Cd+2, Cu+2, In+3, Ni+2, Pd+2, and Zn+2 by in vitro cultures of Balb/c 3T3 fibroblasts as a function of concentration of the cations in cell-culture medium. Reversibility of this uptake was also measured. Metal cations exhibited a 400-fold difference in their tendency to accumulate in the cells; In+3 tended to accumulate the most, whereas Pd+2 accumulated the least. Uptake of the cations in the cells increased linearly with the concentration of the cation in the medium for all cations up to their 50% toxicity concentrations. Reversal of this uptake was slower than that of the initial uptake for three cations studied in more detail (Cd+2, In+3, and Ni+2). The duration of the initial exposure affected the proportion of the metal cations that were retained by the cells 5 h after the cations were removed from the medium. The proportion of retained Cd+2 did not change when the initial exposure was increased from 2 h to 6 h, whereas the proportion of retained In+3 decreased and Ni+2 increased over the same period. The tendency of the cells to accumulate these cations correlated with their cytotoxic potency (measured previously).
采用原子吸收光谱法评估体外培养的Balb/c 3T3成纤维细胞对Ag+1、Au+4、Cd+2、Cu+2、In+3、Ni+2、Pd+2和Zn+2的摄取情况，该摄取情况是细胞培养基中阳离子浓度的函数。还测量了这种摄取的可逆性。金属阳离子在细胞内积累的倾向存在400倍的差异；In+3积累最多，而Pd+2积累最少。对于所有阳离子，在达到其50%毒性浓度之前，细胞对阳离子的摄取随培养基中阳离子浓度的增加呈线性增加。对于更详细研究的三种阳离子（Cd+2、In+3和Ni+2），这种摄取的逆转比初始摄取要慢。初始暴露的持续时间影响从培养基中去除阳离子5小时后细胞保留的金属阳离子比例。当初始暴露从2小时增加到6小时时，保留的Cd+2比例没有变化，而同期保留的In+3比例下降，Ni+2比例上升。细胞积累这些阳离子的倾向与其细胞毒性效力（先前测量）相关。
