Wataha J C, Hanks C T, Craig R G
University of Michigan School of Dentistry, Ann Arbor 48109-1078.
J Biomed Mater Res. 1994 Apr;28(4):427-33. doi: 10.1002/jbm.820280404.
The effects of Ag+1, Au+3, Cd+2, Cu+2, Ga+3, In+3, Ni+2, Pd+2, and Zn+2 on DNA synthesis, protein synthesis, succinic dehydrogenase activity, and total cellular protein of mammalian fibroblasts were measured for exposures less than 12 h. The rates at which these cellular functions responded to metal ion exposure were compared and related to the uptake rate of the ions into the cells. These rates of response were significantly different: DNA synthesis decreased the fastest, followed by protein synthesis, succinic dehydrogenase activity, and total protein. This order of response was similar for most metal ions. At 4 h, the rate of uptake of the metal ions correlated most closely with depression of succinic dehydrogenase activity, whereas at 8 h, the uptake correlated most closely with depression of protein synthesis. The similar response of cells to all metal ions may imply that these ions act on cells by similar mechanisms. The rates of uptake of Ag+1, Cu+2, and Zn+2 were sufficiently fast that in vivo exposures of tissues to these metals for periods less than 12 h would be capable of disrupting cellular metabolism.
在暴露时间少于12小时的情况下，测定了Ag+1、Au+3、Cd+2、Cu+2、Ga+3、In+3、Ni+2、Pd+2和Zn+2对哺乳动物成纤维细胞DNA合成、蛋白质合成、琥珀酸脱氢酶活性和总细胞蛋白的影响。比较了这些细胞功能对金属离子暴露的反应速率，并将其与离子进入细胞的摄取速率相关联。这些反应速率存在显著差异：DNA合成下降最快，其次是蛋白质合成、琥珀酸脱氢酶活性和总蛋白。大多数金属离子的这种反应顺序相似。在4小时时，金属离子的摄取速率与琥珀酸脱氢酶活性的降低最密切相关，而在8小时时，摄取与蛋白质合成的降低最密切相关。细胞对所有金属离子的相似反应可能意味着这些离子通过相似的机制作用于细胞。Ag+1、Cu+2和Zn+2的摄取速率足够快，以至于组织在体内暴露于这些金属少于12小时就能够破坏细胞代谢。
