Brunstedt M R, Ziats N P, Schubert M, Hiltner P A, Anderson J M, Lodoen G A, Payet C R
Department of Macromolecular Science, Case Western Reserve University, Cleveland, Ohio 44106.
J Biomed Mater Res. 1993 Feb;27(2):255-67. doi: 10.1002/jbm.820270216.
Surface characterization and protein adsorption studies were carried out on a series of additive dispersed and additive coated poly(ether urethane ureas), PEUUs, to characterize early events in the blood compatibility of these materials. A hypothesis that is based on surface hydrophilicity, surface flexibility, and adsorption media has been developed to understand the modulated adsorption of plasma proteins by PEUU additives. Electron spectroscopy for chemical analysis (ESCA) and contact angle analysis were performed on two PEUU formulation as well as on PEUU formulations modified with Methacrol 2138F (co[diisopropylaminoethyl methacrylate (DIPAM)/decyl methacrylate (DM)][3/1]) or acrylate or methacrylate polymer or copolymer analogs of Methacrol 2138F. Methacrol 2138F is a commercially used amphiphilic copolymethacrylate. ESCA showed that the PEUUs loaded with Methacrol 2138F or with its hydrophilic component, homopoly (DIPAM) (h-(DIPAM)), had a higher percentage of nitrogen at their surfaces than did the base PEUUs. Contact angle analysis also showed that the air side of PEUU formulations loaded with Methacrol 2138F were more hydrophobic than was the air side of base PEUUs when films were cast from dimethylacetamide. However, during contact angle testing, the air side of PEUU films loaded with Methacrol 2138F rapidly became more hydrophilic than did the air side of the base PEUU films. A radioimmunoassay and whole or diluted human plasma were also used to characterize the presence of the proteins fibrinogen, immunoglobulin G, factor VIII/von Willebrand factor, Hageman factor (factor XII), and albumin, on the surface of the same PEUUs as analyzed by ESCA and contact angle. The protein adsorption assay showed that PEUU films loaded or coated with Methacrol 2138F, with a copolyacrylate analog of Methacrol 2138F (co(diisopropylaminoethyl acrylate [DIPAA]/decyl acrylate [DA]) [3/1]), or with the hydrophilic polyacrylate or polymethacrylate component analogs of Methacrol 2138F (h-DIPAM or h-DIPAA) adsorbed significantly lower amounts of the proteins than did either the base PEUU formulations or the homopoly(decyl methacrylate) (h-DM) or homopoly(decyl acrylate) (h-DA) coated or loaded PEUUs.
对一系列添加物分散和添加物包覆的聚（醚脲脲）（PEUUs）进行了表面表征和蛋白质吸附研究，以表征这些材料血液相容性的早期事件。基于表面亲水性、表面柔韧性和吸附介质，提出了一个假设，以了解PEUU添加剂对血浆蛋白吸附的调节作用。对两种PEUU配方以及用甲基丙烯酸2138F（共[甲基丙烯酸二异丙氨基乙酯（DIPAM）/甲基丙烯酸癸酯（DM）][3/1]）或丙烯酸酯或甲基丙烯酸酯聚合物或甲基丙烯酸2138F的共聚物类似物改性的PEUU配方进行了化学分析电子能谱（ESCA）和接触角分析。甲基丙烯酸2138F是一种商业上使用的两亲性共聚甲基丙烯酸酯。ESCA表明，负载甲基丙烯酸2138F或其亲水成分均聚（DIPAM）（h-（DIPAM））的PEUUs在其表面的氮含量百分比高于基础PEUUs。接触角分析还表明，当从二甲基乙酰胺浇铸薄膜时，负载甲基丙烯酸2138F的PEUU配方的空气侧比基础PEUUs的空气侧更疏水。然而，在接触角测试期间，负载甲基丙烯酸2138F的PEUU薄膜的空气侧比基础PEUU薄膜的空气侧迅速变得更亲水。还使用放射免疫测定法和全血或稀释的人血浆来表征与通过ESCA和接触角分析的相同PEUUs表面上纤维蛋白原、免疫球蛋白G、因子VIII/血管性血友病因子、哈格曼因子（因子XII）和白蛋白的存在情况。蛋白质吸附试验表明，负载或包覆甲基丙烯酸2138F、甲基丙烯酸2138F的丙烯酸酯共聚物类似物（共[丙烯酸二异丙氨基乙酯（DIPAA）/丙烯酸癸酯（DA）][3/1]）或甲基丙烯酸2138F的亲水性聚丙烯酸酯或聚甲基丙烯酸酯成分类似物（h-DIPAM或h-DIPAA）的PEUU薄膜吸附的蛋白质数量明显低于基础PEUU配方或包覆或负载均聚（甲基丙烯酸癸酯）（h-DM）或均聚（丙烯酸癸酯）（h-DA）的PEUUs。
