Pérez M D, Puyol P, Ena J M, Calvo M
Tecnología y Bioquímica de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, España.
J Dairy Res. 1993 Feb;60(1):55-63. doi: 10.1017/s0022029900027345.
The interaction of sheep, horse, pig, human and guinea-pig whey proteins with fatty acids has been studied. Using gel filtration and autoradiography, it was found that sheep beta-lactoglobulin and serum albumin from all species had the ability to bind fatty acids in vitro. Sheep beta-lactoglobulin, isolated from milk, had approximately 0.5 mol fatty acids bound per mol monomer protein, and albumin from sheep, horse and pig contained approximately 4.5, 2.9 and 4.7 mol fatty acids/mol protein respectively. However, beta-lactoglobulin from horse and pig milk had neither fatty acids physiologically bound nor the ability to bind them in vitro. Albumin was the only whey protein detected with bound fatty acids in these species as well as in human and guinea pig. This suggests that the ability of ruminant beta-lactoglobulin to bind fatty acids was not shared by the same protein of non-ruminants.
已对绵羊、马、猪、人类和豚鼠的乳清蛋白与脂肪酸的相互作用进行了研究。通过凝胶过滤和放射自显影发现，绵羊β-乳球蛋白以及所有物种的血清白蛋白在体外均具有结合脂肪酸的能力。从牛奶中分离出的绵羊β-乳球蛋白，每摩尔单体蛋白约结合0.5摩尔脂肪酸，绵羊、马和猪的白蛋白分别含有约4.5、2.9和4.7摩尔脂肪酸/摩尔蛋白。然而，马奶和猪奶中的β-乳球蛋白既没有生理结合的脂肪酸，也没有在体外结合脂肪酸的能力。在这些物种以及人类和豚鼠中，白蛋白是唯一检测到有结合脂肪酸的乳清蛋白。这表明反刍动物β-乳球蛋白结合脂肪酸的能力并非非反刍动物的同一种蛋白所具备。
