Popko B, Goodrum J F, Bouldin T W, Zhang S H, Maeda N
Brain and Development Research Center, University of North Carolina, Chapel Hill 27599.
J Neurochem. 1993 Mar;60(3):1155-8. doi: 10.1111/j.1471-4159.1993.tb03268.x.
The concentration of apolipoprotein E (apoE), a high-affinity ligand for the low-density lipoprotein receptor, increases dramatically in peripheral nerve following injury. This endoneurial apoE is thought to play an important role in the redistribution of lipids from the degenerating axonal and myelin membranes to the regenerating axons and myelin sheaths. The importance of apoE in nerve repair was examined using mutant mice that lack apoE. We show that at 2 and 4 weeks following sciatic nerve crush, regenerating nerves in apoE-deficient mice were morphologically similar to regenerating nerves in control animals, indicating that apoE is not essential for peripheral nerve repair. Moreover, cholesterol synthesis was reduced in regenerating nerves of apoE-deficient mice as much as in regenerating nerves of control animals. These results suggest that the intraneural conservation and reutilization of cholesterol following nerve injury do not require apoE.
载脂蛋白E（apoE）是低密度脂蛋白受体的高亲和力配体，其在外周神经损伤后浓度急剧增加。这种神经内膜apoE被认为在脂质从退变的轴突和髓鞘膜重新分布到再生轴突和髓鞘中起重要作用。使用缺乏apoE的突变小鼠研究了apoE在神经修复中的重要性。我们发现，在坐骨神经挤压伤后2周和4周，apoE缺陷小鼠的再生神经在形态上与对照动物的再生神经相似，表明apoE对于外周神经修复并非必不可少。此外，apoE缺陷小鼠再生神经中的胆固醇合成减少程度与对照动物再生神经中的相同。这些结果表明，神经损伤后神经内胆固醇的保存和再利用不需要apoE。
