Aimoto T, Onda M, Uchida E, Asano G
First Department of Surgery, Nippon Medical School, Tokyo, Japan.
Nihon Geka Gakkai Zasshi. 1993 Jan;94(1):78-85.
NORs (Nucleolar Organizer Regions) are loops of DNA encoding ribosomal RNA, which reflect cellular activity. The Ag-NORs staining was applied to paraffin sections of 10 normal pancreas, 10 chronic pancreatitis and 23 pancreatic cancer, with 17 gastric and 18 colonic cancers. We calculated the mean Ag-NORs number and Ag-NORs index, and defined T/N ratio. The mean Ag-NORs number was compared with the data obtained by Flow cytometry (FCM) in pancreatic carcinoma. A significant difference in Ag-NORs index was found between benign (normal: 1.86 +/- 0.38, pancreatitis: 2.14 +/- 0.53) and malignant tissues (4.02 +/- 1.19) of the pancreas (p < 0.01). Compared with the data by FCM, there was a significant correlation between the mean Ag-NORs number and the percentage of S-phase cells in pancreatic carcinoma (r = 0.59, p < 0.01). Ag-NORs index in pancreatic carcinoma showed significantly lower value than that in cancer of stomach (6.35 +/- 1.32) and colon (7.66 +/- 1.35), but there was no difference in T/N ratio among them. In conclusion, the Ag-NORs staining is useful for analyzing proliferating activity in pancreatic carcinoma. The proliferating potential in pancreatic carcinoma is lower than that in gastric and colonic ones. This may be due to the low proliferating potential of the background mucosa from which pancreatic carcinoma will arise.
核仁组织区（NORs）是编码核糖体RNA的DNA环，反映细胞活性。将银染核仁组织区（Ag-NORs）染色应用于10例正常胰腺、10例慢性胰腺炎和23例胰腺癌的石蜡切片，并与17例胃癌和18例结肠癌进行对比。我们计算了平均Ag-NORs数量和Ag-NORs指数，并定义了T/N比值。将胰腺癌中平均Ag-NORs数量与通过流式细胞术（FCM）获得的数据进行比较。发现胰腺良性组织（正常：1.86±0.38，胰腺炎：2.14±0.53）和恶性组织（4.02±1.19）之间的Ag-NORs指数存在显著差异（p<0.01）。与FCM数据相比，胰腺癌中平均Ag-NORs数量与S期细胞百分比之间存在显著相关性（r = 0.59，p<0.01）。胰腺癌的Ag-NORs指数显著低于胃癌（6.35±1.32）和结肠癌（7.66±1.35），但它们之间的T/N比值没有差异。总之，Ag-NORs染色有助于分析胰腺癌的增殖活性。胰腺癌的增殖潜能低于胃癌和结肠癌。这可能是由于胰腺癌起源的背景黏膜增殖潜能较低。
