Vlatakis G, Andersson L I, Müller R, Mosbach K
Department of Pure and Applied Biochemistry, University of Lund, Sweden.
Nature. 1993 Feb 18;361(6413):645-7. doi: 10.1038/361645a0.
Ligand-binding assays are used for determination of minute amounts of substances in the bloodstream. Such assays require a receptor that specifically binds the substance of interest. The receptor used is often an antibody, but antibodies require special handling and a costly production procedure. We have used molecular imprinting, a method for creating selective recognition sites in synthetic polymers, to prepare polymers that mimic antibody combining sites. Molecular imprints made against theophylline and diazepam showed strong binding and cross-reactivity profiles similar to those of antibodies. Here we describe a new radiolabelled ligand-binding assay, the molecularly imprinted sorbent assay, which uses antibody mimics. This assay accurately measures drug levels in human serum, with results comparable to those obtained using a well established immunoassay technique. Antibody mimics, which are stable and readily prepared by molecular imprinting, may provide a useful general alternative to antibodies.
配体结合分析用于测定血液中的微量物质。此类分析需要一种能特异性结合目标物质的受体。所使用的受体通常是抗体，但抗体需要特殊处理且生产过程成本高昂。我们利用分子印迹技术（一种在合成聚合物中创建选择性识别位点的方法）制备了模拟抗体结合位点的聚合物。针对茶碱和地西泮制备的分子印迹聚合物表现出与抗体相似的强结合力和交叉反应谱。在此，我们描述一种新的放射性标记配体结合分析方法——分子印迹吸附剂分析，该方法使用抗体模拟物。这种分析方法能准确测量人血清中的药物水平，其结果与使用成熟免疫分析技术获得的结果相当。通过分子印迹技术稳定且易于制备的抗体模拟物，可能为抗体提供一种有用的通用替代物。
