Guilhot C, Benchaibi M, Flechon J E, Samarut J
Laboratoire de Biologie Moléculaire et Cellulaire, CNRS UMR49, INRA, Ecole Normale Supérieure de Lyon, France.
Oncogene. 1993 Mar;8(3):619-24.
Quail cells were immortalized for the first time by using retroviruses expressing the 12S adenoviral E1A gene. In these cells, interaction between the 12S E1A product and the quail RB protein was shown, suggesting that the 12S adenoviral E1A product works in avian cells through similar biochemical pathways as in mammalian cells by interacting and inactivating host cellular proteins, including the RB product. These results confirm that the RB product exhibits a universal function among higher vertebrates in controlling cellular growth and tumor progression.
通过使用表达12S腺病毒E1A基因的逆转录病毒，鹌鹑细胞首次实现了永生化。在这些细胞中，显示出12S E1A产物与鹌鹑RB蛋白之间的相互作用，这表明12S腺病毒E1A产物在禽类细胞中通过与包括RB产物在内的宿主细胞蛋白相互作用并使其失活，从而通过与哺乳动物细胞中相似的生化途径发挥作用。这些结果证实，RB产物在高等脊椎动物中在控制细胞生长和肿瘤进展方面具有普遍功能。
