Charles C H, Yoon J K, Simske J S, Lau L F
Department of Genetics, University of Illinois College of Medicine, Chicago 60612.
Oncogene. 1993 Mar;8(3):797-801.
We report the cDNA sequence and genomic structure of gly96, an immediate early gene inducible by serum growth factors in mouse fibroblasts. It encodes a 153-amino acid protein that does not share significant sequence similarity with any known protein. In the adult mouse, gly96 is expressed predominantly in the lung, testes and the uterus. We have identified the Gly96 protein in Balb/c 3T3 cells using affinity-purified antibodies recognizing the Gly96 polypeptide. We show that Gly96 is glycosylated and has a short half-life in serum stimulated fibroblasts.
我们报道了gly96的cDNA序列和基因组结构，gly96是小鼠成纤维细胞中一种可被血清生长因子诱导的 immediate early 基因。它编码一种153个氨基酸的蛋白质，该蛋白质与任何已知蛋白质都没有明显的序列相似性。在成年小鼠中，gly96主要在肺、睾丸和子宫中表达。我们使用识别Gly96多肽的亲和纯化抗体在Balb/c 3T3细胞中鉴定出了Gly96蛋白。我们发现Gly96是糖基化的，并且在血清刺激的成纤维细胞中的半衰期较短。
