Quant P A
Department of Biochemistry, University of Cambridge, UK.
Trends Biochem Sci. 1993 Jan;18(1):26-30. doi: 10.1016/0968-0004(93)90084-z.
Metabolic control analysis (MCA) has provided the language and framework for quantitative study of control over flux, or over metabolites, by individual enzymes of a pathway. By contrast, top-down control analysis (TDCA) yields an immediate overview of the control structure of the whole system of interest, giving information about the control exercised by large sections of complex pathways. Unlike MCA, TDCA does not rely on the use of specific inhibitors or genetic manipulation to determine control coefficients. The method and an application of TDCA to ketogenesis are described.
代谢控制分析（MCA）为定量研究途径中的单个酶对通量或代谢物的控制提供了语言和框架。相比之下，自上而下控制分析（TDCA）能立即对整个感兴趣系统的控制结构进行概述，给出关于复杂途径中大部分区域所行使控制的信息。与MCA不同，TDCA不依赖于使用特定抑制剂或基因操作来确定控制系数。本文描述了TDCA方法及其在生酮作用中的应用。
