Gessani S, Dieffenbach C W, Conti L, DiMarzio P, Wilson K L, Belardelli F
Department of Virology, Istituto Superiore di Sanità, Rome, Italy.
Virology. 1993 Mar;193(1):507-9. doi: 10.1006/viro.1993.1154.
Basal levels of interferon (IFN)-beta mRNA transcription were detected in both freshly explanted LPS-responsive (Lpsn) and LPS-hyporesponsive (Lpsd) peritoneal macrophages (PM). In vitro cultivation of PM resulted in a time-dependent reduction in the level of IFN-beta mRNA, which was far more rapid in Lpsd than in Lpsn PM. Treatment of Lpsn PM with cycloheximide (CHX) resulted in a marked accumulation of IFN-beta mRNA, which was not associated with an increase in IFN-beta gene transcription. However, treatment of Lpsd PM with CHX did not induce accumulation of IFN-beta mRNA. CHX induced the accumulation of IFN-alpha-4 mRNA in both Lpsn and Lpsd PM, CHX enhanced the accumulation of two cytoplasmic factors interacting with AU-rich sequences within the 3' untranslated region of IFN-beta mRNA. We conclude that Lpsd PM exhibit an impaired capacity to stabilize IFN-beta mRNA that may account for their low expression of IFN-beta.
在新鲜分离的对脂多糖（LPS）有反应（Lpsn）和对LPS反应低下（Lpsd）的腹腔巨噬细胞（PM）中均检测到干扰素（IFN）-β mRNA转录的基础水平。PM的体外培养导致IFN-β mRNA水平呈时间依赖性降低，Lpsd中的降低速度比Lpsn PM快得多。用环己酰亚胺（CHX）处理Lpsn PM导致IFN-β mRNA显著积累，这与IFN-β基因转录增加无关。然而，用CHX处理Lpsd PM并未诱导IFN-β mRNA积累。CHX诱导Lpsn和Lpsd PM中IFN-α-4 mRNA积累，CHX增强了两种与IFN-β mRNA 3'非翻译区内富含AU序列相互作用的细胞质因子的积累。我们得出结论，Lpsd PM稳定IFN-β mRNA的能力受损，这可能是其IFN-β低表达的原因。
