Horny H P, Wehrmann M, Griesser H, Tiemann M, Bültmann B, Kaiserling E
Institute of Pathology, University of Tübingen, Germany.
Am J Clin Pathol. 1993 Feb;99(2):142-9. doi: 10.1093/ajcp/99.2.142.
Bone marrow lymphocyte subsets in normal and reactive states and in neoplastic diseases involving the marrow were investigated with a select panel of monoclonal antibodies reactive on routinely processed, paraffin-embedded trephine biopsy material. In all cases, the antibodies beta F1 and UCHL1 (CD45RO) stained virtually equal numbers of T cells (reactive and neoplastic), whereas antibody OPD4 stained only about one half of this number of T cells. Antibody L26 (CD20) stained B cells (reactive and neoplastic) in all specimens. The T-cell to B-cell ratio in the normal marrow was between 4:1 and 5:1, and a significant increase in T-cell numbers was observed in reactive and myelodysplastic states. A significant increase in B-cell numbers, however, was seen only in marrow infiltrated by B-cell lymphoma. Bone marrow exhibiting infiltrates of B-cell lymphoma, acute leukemia, or myeloproliferative disorders showed normal or decreased numbers of T cells. These findings show that antibodies UCHL1, beta F1, and L26 can be used to determine the numbers of B and T lymphocytes in paraffin-embedded, formalin-fixed bone marrow specimens and thus may help to distinguish reactive T lymphocytosis from B-cell lymphoma.
利用一组对常规处理的石蜡包埋环钻活检材料有反应性的单克隆抗体，对正常、反应性状态以及累及骨髓的肿瘤性疾病中的骨髓淋巴细胞亚群进行了研究。在所有病例中，抗体βF1和UCHL1（CD45RO）染色的T细胞（反应性和肿瘤性）数量几乎相等，而抗体OPD4染色的T细胞数量仅约为前者的一半。抗体L26（CD20）在所有标本中均能染色B细胞（反应性和肿瘤性）。正常骨髓中T细胞与B细胞的比例在4:1至5:1之间，在反应性和骨髓增生异常状态下观察到T细胞数量显著增加。然而，仅在B细胞淋巴瘤浸润的骨髓中可见B细胞数量显著增加。表现为B细胞淋巴瘤、急性白血病或骨髓增殖性疾病浸润的骨髓，其T细胞数量正常或减少。这些发现表明，抗体UCHL1、βF1和L26可用于确定石蜡包埋、福尔马林固定的骨髓标本中B淋巴细胞和T淋巴细胞的数量，从而可能有助于区分反应性T淋巴细胞增多症与B细胞淋巴瘤。
