非洲爪蟾前神经肽Y原编码下丘脑cDNA的克隆与序列分析
Cloning and sequence analysis of hypothalamic cDNA encoding Xenopus preproneuropeptide Y.
作者信息
van Riel M C, Tuinhof R, Roubos E W, Martens G J
机构信息
Department of Animal Physiology, University of Nijmegen, The Netherlands.
出版信息
Biochem Biophys Res Commun. 1993 Feb 15;190(3):948-51. doi: 10.1006/bbrc.1993.1141.
Neuropeptide Y (NPY) consists of 36 amino acids and it constitutes one of the most conserved neuropeptides. Here we report the complete sequence of the first amphibian NPY precursor by cloning of a hypothalamic cDNA encoding Xenopus laevis preproNPY. The overall amino acid sequence identity between Xenopus and other known NPY precursor proteins ranges from 59% (fish) to 82% (chicken); a low degree of identity was found for the signal peptide sequence (32-75%) and for the carboxy-terminal peptide of NPY (CPON; 43-73%), while the NPY peptide sequence itself constitutes the most highly-conserved region (89-100%) within the preproNPY structure.
神经肽Y（NPY）由36个氨基酸组成，是最保守的神经肽之一。在此，我们通过克隆编码非洲爪蟾前神经肽Y（preproNPY）的下丘脑cDNA，报告了首个两栖动物NPY前体的完整序列。非洲爪蟾与其他已知NPY前体蛋白之间的总体氨基酸序列同一性在59%（鱼类）至82%（鸡）之间；信号肽序列（32 - 75%）和NPY的羧基末端肽（CPON；43 - 73%）的同一性较低，而NPY肽序列本身是前神经肽Y结构中保守性最高的区域（89 - 100%）。
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