Winkelhake J L, Elcombe B M, Chang R J
Physiol Chem Phys. 1978;10(4):305-22.
Several macromolecular homeostasis-regulating mechanisms were tested for functional integrities in mice during acute and early chronic phases of infection with lactic dehydrogenase-elevating virus (LDV). Fractional catabolic rates of carbodiimide-aggregated albumin and immunoglobulin G were studied to evaluate glomerular filtration and hepatic Kupffer cell phagocytic activities. Several glycoproteins (fetuin, IgG antibodies, and ovalbumin) were also compared with their deglycosylated counterparts for fractional catabolic rates and organ distributions as a basis for evaluating virus-induced modifications of saccharide-binding "receptor functions" in vivo. Findings were that normal hepatic clearance of aggregated albumin and of ovalbumin is slowed from the onset of viremia. Fractional catabolic rates of amannosyl-ovalbumin and amannosyl-IgG are similar in uninfected animals to those seen with native ovalbumin or with mannose-terminated IgG in LDV-infected animals. Ovalbumin and aggregated albumin were also found to be mutually competitive for hepatic uptake in uninfected animals. It is proposed that LDV, which replicates in cells of the mononuclear phagocyte system (reticuloendothelial system), alters the clearance functional state of fixed tissue macrophage, thereby explaining in part the protracted circulatory longevity of several enzymes, aggregated albumin and mannose-terminated ovalbumin, and IgG in LDV-infected mice.
在感染乳酸脱氢酶升高病毒（LDV）的急性和早期慢性阶段，对小鼠体内几种大分子稳态调节机制的功能完整性进行了测试。研究了碳二亚胺聚集白蛋白和免疫球蛋白G的分解代谢率，以评估肾小球滤过和肝库普弗细胞的吞噬活性。还比较了几种糖蛋白（胎球蛋白、IgG抗体和卵清蛋白）与其去糖基化对应物的分解代谢率和器官分布，作为评估病毒在体内诱导的糖结合“受体功能”修饰的基础。研究结果表明，从病毒血症开始，聚集白蛋白和卵清蛋白的正常肝脏清除率就会减慢。在未感染的动物中，去甘露糖基化卵清蛋白和去甘露糖基化IgG的分解代谢率与LDV感染动物中天然卵清蛋白或甘露糖末端IgG的分解代谢率相似。在未感染的动物中，还发现卵清蛋白和聚集白蛋白在肝脏摄取方面相互竞争。有人提出，在单核吞噬细胞系统（网状内皮系统）细胞中复制的LDV会改变固定组织巨噬细胞的清除功能状态，从而部分解释了LDV感染小鼠体内几种酶、聚集白蛋白和甘露糖末端卵清蛋白以及IgG循环寿命延长的原因。
