Ogura A, Yanagimachi R
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
Biol Reprod. 1993 Feb;48(2):219-25. doi: 10.1095/biolreprod48.2.219.
Round spermatids are spermatogenic cells that have just completed meiosis. To discover whether nuclei of these haploid cells are genetically ready for fertilization, nuclei were individually injected into mature hamster oocytes via a microsurgical technique. In the majority of oocytes that were successfully injected and activated, spermatid nuclei transformed into pronuclei and underwent DNA synthesis. Ultimately their chromosomes mingled with ootid chromosomes immediately prior to the first cleavage. Although we could not determine the developmental potential of these zygotes, spermatid nuclei appear capable of participating in syngamy.
圆形精子细胞是刚完成减数分裂的生精细胞。为了探究这些单倍体细胞的细胞核在遗传上是否已准备好进行受精，通过显微手术技术将细胞核逐个注入成熟的仓鼠卵母细胞中。在大多数成功注射并激活的卵母细胞中，精子细胞核转变为原核并进行DNA合成。最终，它们的染色体在第一次分裂前立即与卵核染色体混合。尽管我们无法确定这些合子的发育潜能，但精子细胞核似乎有能力参与配子融合。
