磷脂酰胆碱在大鼠脑突触质膜中的不对称分布。
The asymmetric distribution of phosphatidylcholine in rat brain synaptic plasma membranes.
作者信息
Shina R, Crain R C, Rosenberg P, Condrea E
机构信息
Basil and Gerald Felsenstein Medical Research Center, Rogoff-Wellcome Medical Research Institute, Sackler School of Medicine, Tel Aviv University, Beilinson Medical Center, Petah Tikva, Israel.
出版信息
Neurochem Int. 1993 Feb;22(2):189-95. doi: 10.1016/0197-0186(93)90012-t.
The distribution of phosphatidylcholine between inner and outer monolayers of rat brain synaptic plasma membrane was investigated by means of a phosphatidylcholine specific exchange protein. About 70% of the total membranal phosphatidylcholine was in the outer leaflet, 33% of which was exposed and readily exchanged in intact synaptosomes while the remainder was exchangeable following osmotic shock. Permeabilization of the synaptic plasma membranes by overnight incubation in buffer or by saponin (< 0.08%) exposed an additional 30% of phosphatidylcholine to exchange, presumably from the inner cytoplasmic leaflet. Phosphatidylcholine is therefore asymmetrically distributed in the synaptosomal plasma membrane, as it is in other plasma membranes.
利用磷脂酰胆碱特异性交换蛋白研究了大鼠脑突触质膜内外单层之间磷脂酰胆碱的分布情况。膜结合的总磷脂酰胆碱中约70%位于外层小叶，其中33%在完整突触体中暴露并易于交换，其余部分在渗透休克后可进行交换。通过在缓冲液中过夜孵育或用皂素（<0.08%）使突触质膜通透，可使另外30%的磷脂酰胆碱暴露以进行交换，推测这些磷脂酰胆碱来自内层细胞质小叶。因此，磷脂酰胆碱在突触体质膜中呈不对称分布，就像在其他质膜中一样。
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