一种在非放射性原位杂交后使用的简单G显带技术。
A simple G-banding technique following nonradioactive in situ hybridization.
作者信息
Duncan L M, Dietzsch E, Rudduck C, Garson O M
机构信息
Department of Medicine, University of Melbourne, Victoria, Australia.
出版信息
Cytogenet Cell Genet. 1993;62(4):228-30. doi: 10.1159/000133483.
PMID:8440141
Abstract
A simple G-banding protocol has been found to be compatible with a nonisotopic in situ hybridization technique utilizing a biotin-streptavidin-polyalkaline phosphatase complex. The chromosomes are banded following detection of the hybridization signal, the procedure requires no pretreatment of cultures or special optical equipment, and it produces permanent preparations.
摘要
已发现一种简单的G显带方案与利用生物素-链霉亲和素-多碱性磷酸酶复合物的非同位素原位杂交技术兼容。在检测到杂交信号后对染色体进行显带，该程序不需要对培养物进行预处理或特殊光学设备，并且能产生永久性制片。
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