Lindberg W, Ruzicka J, Christian G D
Department of Chemistry, University of Washington, Seattle 98195.
Cytometry. 1993;14(2):230-6. doi: 10.1002/cyto.990140217.
A prototype instrument for sample and solution handling in flow cytometry has been constructed. The system is modular and allows the control of any combination of up to 4 pumps, 2 selection valves, and 2 injection valves. These devices are controlled by a computer using TTL-logic. The flow injection instrument is interfaced to the flow cytometer via a 6-port injection valve, thereby facilitating virtually any flow pattern to be implemented without affecting the fluidics of the cytometer. Salient features of the instrument are accurate control of the volume of injected sample and reagent, reproducible timing, and controlled mixing conditions. Results from model experiments of on-line staining of trout erythrocytes with different concentrations of 4',6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) are shown. Possible ways to improve the performance and utility of the instrument are also discussed.
已构建了一种用于流式细胞术中样本和溶液处理的原型仪器。该系统是模块化的，可控制多达4个泵、2个选择阀和2个进样阀的任意组合。这些设备由一台使用TTL逻辑的计算机控制。流动注射仪器通过一个6端口进样阀与流式细胞仪相连，从而便于在不影响细胞仪流体系统的情况下实现几乎任何流动模式。该仪器的显著特点是对注入样本和试剂的体积进行精确控制、定时可重复以及混合条件可控。展示了用不同浓度的4',6-二脒基-2-苯基吲哚（DAPI）和碘化丙啶（PI）对虹鳟红细胞进行在线染色的模型实验结果。还讨论了提高该仪器性能和实用性的可能方法。
