Omann G M, Coppersmith W, Finney D A, Sklar L A
Cytometry. 1985 Jan;6(1):69-73. doi: 10.1002/cyto.990060113.
We describe a simple and inexpensive device which permits the addition of up to three different solutions into a cell suspension which is on-line in a flow cytometer. The mixing chamber houses a disposable plastic cuvette stirred with a magnetic stirrer. The sample chamber is attached to a circulating water bath, hence accurate temperature control is achieved. Because the system is prepressurized and the sample line is very short, the delay time-between the point of sample modification and the point of analysis is reduced to a few seconds. Thus reagents may be added rapidly, and kinetic measurements of high temporal resolution are possible. Because the temperature of the sample chamber is regulated, binding can be observed over longer time periods than was previously possible. We demonstrate the usefulness of this device in determining the binding of fluoresceinated hexapeptide to human neutrophils under conditions where the stimulus is infused into the cell suspension while on-line in the cytometer.
我们描述了一种简单且成本低廉的装置，它能够将多达三种不同的溶液添加到流式细胞仪在线检测的细胞悬液中。混合室中放置着一个由磁力搅拌器搅拌的一次性塑料比色皿。样品室连接到循环水浴，从而实现精确的温度控制。由于该系统预先加压且样品管路非常短，样品修饰点与分析点之间的延迟时间缩短至几秒。因此，可以快速添加试剂，并进行高时间分辨率的动力学测量。由于样品室的温度受到调节，所以能够在比以往更长的时间段内观察结合情况。我们展示了该装置在以下条件下的实用性：在流式细胞仪在线检测时，将刺激物注入细胞悬液，以此来测定荧光素化六肽与人中性粒细胞的结合情况。
