维生素B6生物合成受阻的大肠杆菌突变体对4-羟基-L-苏氨酸的生长反应。

Growth response to 4-hydroxy-L-threonine of Escherichia coli mutants blocked in vitamin B6 biosynthesis.

作者信息

Drewke C, Notheis C, Hansen U, Leistner E, Hemscheidt T, Hill R E, Spenser I D

机构信息

Institut für Pharmazeutische Biologie, Universität Bonn, Germany.

出版信息

FEBS Lett. 1993 Mar 1;318(2):125-8. doi: 10.1016/0014-5793(93)80005-f.

DOI:10.1016/0014-5793(93)80005-f

PMID:8440369

Abstract

Mutants of Escherichia coli (pdx B and pdx C) which are blocked in the biosynthesis of pyridoxol (vitamin B6) showed a growth response to 4-hydroxy-L-threonine. This observation constitutes the first direct evidence in support of the view that 4-hydroxy-L-threonine is implicated in the biosynthesis of vitamin B6. 1-Aminopropan-2,3-diol, the decarboxylation product of 4-hydroxy-L-threonine, does not support the growth of these mutants. Deuterium from deuterium-labelled 1-aminopropan-2,3-diol was not incorporated into pyridoxol.

摘要

在吡哆醇（维生素B6）生物合成过程中受阻的大肠杆菌突变体（pdx B和pdx C）对4-羟基-L-苏氨酸表现出生长反应。这一观察结果构成了首个直接证据，支持4-羟基-L-苏氨酸参与维生素B6生物合成的观点。4-羟基-L-苏氨酸的脱羧产物1-氨基丙-2,3-二醇不能支持这些突变体的生长。来自氘标记的1-氨基丙-2,3-二醇的氘未掺入吡哆醇中。