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大肠杆菌K-12中由gapA和epd（gapB）编码的脱氢酶参与磷酸吡哆醛辅酶生物合成

Involvement of the gapA- and epd (gapB)-encoded dehydrogenases in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12.

作者信息

Yang Y, Zhao G, Man T K, Winkler M E

机构信息

Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030-1501, USA.

出版信息

J Bacteriol. 1998 Aug;180(16):4294-9. doi: 10.1128/JB.180.16.4294-4299.1998.

DOI:10.1128/JB.180.16.4294-4299.1998
PMID:9696782
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107430/
Abstract

We show that epd (gapB) mutants lacking an erythrose 4-phosphate (E4P) dehydrogenase are impaired for growth on some media and contain less pyridoxal 5'-phosphate (PLP) and pyridoxamine 5'-phosphate (PMP) than their epd+ parent. In contrast to a previous report, we found that gapA epd double mutants lacking the glyceraldehyde 3-phosphate and E4P dehydrogenases are auxotrophic for pyridoxine. These results implicate the GapA and Epd dehydrogenases in de novo PLP and PMP coenzyme biosynthesis.

摘要

我们发现，缺乏磷酸赤藓糖（E4P）脱氢酶的epd（gapB）突变体在某些培养基上生长受损，并且与它们的epd+亲本相比，所含的磷酸吡哆醛（PLP）和磷酸吡哆胺（PMP）更少。与之前的一份报告相反，我们发现缺乏3-磷酸甘油醛和E4P脱氢酶的gapA epd双突变体对吡哆醇营养缺陷。这些结果表明GapA和Epd脱氢酶参与了从头合成PLP和PMP辅酶的过程。

相似文献

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Involvement of the gapA- and epd (gapB)-encoded dehydrogenases in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12.大肠杆菌K-12中由gapA和epd（gapB）编码的脱氢酶参与磷酸吡哆醛辅酶生物合成
J Bacteriol. 1998 Aug;180(16):4294-9. doi: 10.1128/JB.180.16.4294-4299.1998.
2
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本文引用的文献

1
Identification and function of the pdxY gene, which encodes a novel pyridoxal kinase involved in the salvage pathway of pyridoxal 5'-phosphate biosynthesis in Escherichia coli K-12.pdxY基因的鉴定与功能，该基因编码一种新型吡哆醛激酶，参与大肠杆菌K-12中5'-磷酸吡哆醛生物合成的补救途径。
J Bacteriol. 1998 Apr;180(7):1814-21. doi: 10.1128/JB.180.7.1814-1821.1998.
2
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J Bacteriol. 1997 Aug;179(16):5218-21. doi: 10.1128/jb.179.16.5218-5221.1997.
3
Comparative enzymatic properties of GapB-encoded erythrose-4-phosphate dehydrogenase of Escherichia coli and phosphorylating glyceraldehyde-3-phosphate dehydrogenase.大肠杆菌中由GapB编码的4-磷酸赤藓糖脱氢酶与磷酸化3-磷酸甘油醛脱氢酶的比较酶学性质
J Biol Chem. 1997 Jun 13;272(24):15106-12. doi: 10.1074/jbc.272.24.15106.
4
Maximization of transcription of the serC (pdxF)-aroA multifunctional operon by antagonistic effects of the cyclic AMP (cAMP) receptor protein-cAMP complex and Lrp global regulators of Escherichia coli K-12.环腺苷酸（cAMP）受体蛋白-cAMP复合物与大肠杆菌K-12的全局调控因子Lrp的拮抗作用使serC（pdxF）-aroA多功能操纵子的转录最大化。
J Bacteriol. 1997 Jun;179(11):3458-69. doi: 10.1128/jb.179.11.3458-3469.1997.
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A second Escherichia coli gene with similarity to gapA.第二个与gapA基因相似的大肠杆菌基因。
Microbiologia. 1996 Mar;12(1):99-106.
6
The biogenetic anatomy of vitamin B6. A 13C NMR investigation of the biosynthesis of pyridoxol in Escherichia coli.维生素B6的生源论解剖学。大肠杆菌中吡哆醇生物合成的13C核磁共振研究。
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FEBS Lett. 1993 Mar 1;318(2):125-8. doi: 10.1016/0014-5793(93)80005-f.