Mönnikes H, Schmidt B G, Taché Y
Center for Ulcer Research and Education, Veteran's Affairs Wadsworth Medical Center, Los Angeles, California.
Gastroenterology. 1993 Mar;104(3):716-23. doi: 10.1016/0016-5085(93)91006-4.
Brain corticotropin-releasing factor (CRF) is involved in stress-induced accelerated colonic transit. Brain sites of action of CRF to stimulate colonic transit were investigated in conscious fed rats.
Bilateral guide cannulae were chronically implanted into the paraventricular nucleus of the hypothalamus (PVN) or central amygdala for peptide microinjection and a catheter into the proximal colon to measure colonic transit.
CRF (0.6 nmol/rat) injected into the PVN reduced colonic transit time by 84% and stimulated fecal pellet output 20-fold, whereas CRF injected into sites outside of the PVN or the central amygdala had no effect. CRF stimulatory action was prevented by chlorisondamine, and atropine methyl nitrate but not by bretylium. The stress of avoiding water by standing on a small cube reduced colonic transit time by 75% and increased fecal output by 7-fold. Bilateral microinjection of CRF antagonist, alpha-helical-CRF, into the PVN abolished the colonic response to stress. The CRF antagonist had no effect on basal colonic transit time in nonstressed rats.
Psychological stress-induced stimulation of colonic motor function in fed rats involves CRF pathways in the PVN.
脑促肾上腺皮质激素释放因子（CRF）参与应激诱导的结肠传输加速。本研究在清醒进食大鼠中探究了CRF刺激结肠传输的脑内作用位点。
将双侧引导套管长期植入下丘脑室旁核（PVN）或中央杏仁核用于肽微量注射，并将导管插入近端结肠以测量结肠传输。
向PVN注射CRF（0.6 nmol/只大鼠）可使结肠传输时间缩短84%，并使粪便颗粒排出量增加20倍，而向PVN或中央杏仁核以外的部位注射CRF则无此作用。氯筒箭毒碱和硝酸甲基阿托品可阻断CRF的刺激作用，但溴苄铵无此作用。通过站在小方块上避免饮水的应激可使结肠传输时间缩短75%，粪便排出量增加7倍。向PVN双侧微量注射CRF拮抗剂α-螺旋CRF可消除结肠对应激的反应。CRF拮抗剂对非应激大鼠的基础结肠传输时间无影响。
心理应激诱导的进食大鼠结肠运动功能刺激涉及PVN中的CRF通路。
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