de Martin R, Strasswimmer J, Philipson L
European Molecular Biology Laboratory, Heidelberg, Germany.
Gene. 1993 Feb 14;124(1):137-8. doi: 10.1016/0378-1119(93)90776-y.
A new luciferase-encoding expression vector was generated by inserting the strong transcription termination signal from the mouse c-mos oncogene upstream from a multiple cloning site. This construct significantly reduced background transcription in NIH3T3 cells and has proven useful in the study of a weak promoter from the murine growth-arrest-specific gene gas-1.
通过将来自小鼠c-mos癌基因的强转录终止信号插入多克隆位点上游,构建了一种新的编码荧光素酶的表达载体。该构建体显著降低了NIH3T3细胞中的背景转录,并已证明在研究小鼠生长停滞特异性基因gas-1的弱启动子方面很有用。