Ethier C R, Coloma F M, de Kater A W, Allingham R R
Institute for Biomedical Engineering, University of Toronto, Ontario, Canada.
Invest Ophthalmol Vis Sci. 1993 Feb;34(2):385-94.
The goal of this study was to develop a new technique to deliver drugs or other agents to the lumen of the angular aqueous plexus/Schlemm's canal (AAP/SC) while bypassing the trabecular meshwork, thereby gaining insight into AAP/SC inner wall function.
The anterior chamber is held at a small negative pressure and fluid is allowed to flow retrograde from the limbal vessels, through the collector channels, and into the AAP/SC ("retroperfusion"). Facility measurements are combined with histologic and tracer studies in bovine eyes.
(1) Retroperfusion with a saline solution does not alter facility or change outflow pathway morphology; (2) fluid is able to move retrograde from the scleral surface and enter the lumen of the AAP; and (3) retroperfusion with N-ethyl maleimide causes a dose-dependent increase in washout rate and concomitant inner wall breaks.
It is hypothesized that the observed increase in washout is due to leakage of extracellular materials through breaks in the inner wall.
本研究的目的是开发一种新技术，在绕过小梁网的同时将药物或其他制剂输送到角巩膜静脉丛/施莱姆管（AAP/SC）腔内，从而深入了解AAP/SC内壁的功能。
前房保持在小负压状态，使液体从角膜缘血管逆行流动，通过集液管，进入AAP/SC（“逆行灌注”）。在牛眼中将易流度测量与组织学和示踪剂研究相结合。
（1）用盐溶液逆行灌注不会改变易流度或改变流出途径形态；（2）液体能够从巩膜表面逆行移动并进入AAP腔；（3）用N-乙基马来酰亚胺逆行灌注会导致洗脱率呈剂量依赖性增加并伴有内壁破裂。
据推测，观察到的洗脱增加是由于细胞外物质通过内壁破裂处泄漏所致。
