Lindenmayer J M, Kahn M G, Hertzmark E, Epstein D L
Invest Ophthalmol Vis Sci. 1983 Jun;24(6):710-7.
The aqueous outflow system from anterior chamber to Schlemm's canal was examined by electron microscopy in pairs of enucleated macaque and baboon eyes, perfused via the anterior chamber with mock aqueous humor in one eye and the same fluid with added iodoacetamide (IA) or N-ethyl maleimide (NEM) in the other eye. Many details of the electron micrographs were analyzed in a masked manner using a digitizing bit pad and computer, and also using visual evaluation. Both IA and NEM increased aqueous humor outflow facility, but the morphologic changes induced by IA were quantitatively different from those induced by NEM. Intercellular junctions were not affected by IA, but were disrupted by NEM (P less than 0.01). Vacuoles in the endothelial lining of Schlemm's canal tended to increase in area, but not in number, under the influence of IA, whereas they were not so affected by NEM. No loss of extracellular material was observed in either IA- or NEM-treated eyes. The results indicate that the chemical status of cellular-SH groups may influence aqueous humor outflow facility at multiple sites in the outflow pathway.
通过电子显微镜对摘除的猕猴和狒狒双眼进行研究，观察从前房到施莱姆管的房水流出系统。其中一只眼睛经前房灌注模拟房水，另一只眼睛灌注添加了碘乙酰胺（IA）或N - 乙基马来酰亚胺（NEM）的相同液体。使用数字化数位板和计算机以盲法对电子显微镜图像的许多细节进行分析，同时也进行视觉评估。IA和NEM均增加了房水流出易度，但IA诱导的形态学变化在数量上与NEM诱导的不同。细胞间连接不受IA影响，但被NEM破坏（P小于0.01）。在IA的影响下，施莱姆管内皮衬里的液泡面积倾向于增加，但数量不变，而NEM对其没有这种影响。在IA或NEM处理的眼中均未观察到细胞外物质的丢失。结果表明，细胞-SH基团的化学状态可能在流出途径的多个部位影响房水流出易度。
