Haataja S, Tikkanen K, Liukkonen J, François-Gerard C, Finne J
Department of Medical Biochemistry, University of Turku, Finland.
J Biol Chem. 1993 Feb 25;268(6):4311-7.
Streptococcus suis causes sepsis, meningitis, and other serious infections in piglets, and meningitis in humans. Hemagglutination inhibition experiments with mono- and oligosaccharides and glycoproteins indicated that galactose-binding strains of S. suis recognized the Gal alpha 1-4Gal sequence present in the P1 and Pk blood group antigen structures. In thin-layer chromatography overlay assays the bacteria bound to trihexosylceramide (GbO3) but not to globoside (GbO4) or Forssman glycolipid (GbO5), in contrast to P-fimbriated Escherichia coli, which bound only to the latter two. The S. suis adhesin also differed from that of E. coli in that some of the hydrogen bonds formed with the receptor, as determined with chemically modified receptor analogues, were different. In agreement with the binding specificity, the S. suis bacteria agglutinated best among P blood group erythrocytes those of the P1k and P2k type, and from different animal erythrocytes those from rabbit, which express GbO3 as the predominant glycolipid. Binding to frozen sections of pig pharyngeal tissue was decreased by the free GbO3 oligosaccharide and its protein conjugate, which indicated that the corresponding glycolipid may function as receptor for galactose-binding strains of S. suis in pig pharyngeal epithelium.
猪链球菌可导致仔猪败血症、脑膜炎及其他严重感染，也可引发人类脑膜炎。对单糖、寡糖和糖蛋白进行的血凝抑制试验表明，猪链球菌的半乳糖结合菌株可识别存在于P1和Pk血型抗原结构中的Galα1-4Gal序列。在薄层层析覆盖分析中，与P菌毛化大肠杆菌仅结合后两者不同，该细菌与三己糖神经酰胺（GbO3）结合，但不与球苷脂（GbO4）或福斯曼糖脂（GbO5）结合。猪链球菌黏附素与大肠杆菌黏附素也有所不同，通过化学修饰的受体类似物确定，其与受体形成的一些氢键不同。与结合特异性一致，猪链球菌在P血型红细胞中，对P1k和P2k型红细胞凝集性最佳，在不同动物红细胞中，对以GbO3作为主要糖脂的兔红细胞凝集性最佳。游离的GbO3寡糖及其蛋白缀合物可降低猪链球菌与猪咽组织冰冻切片的结合，这表明相应的糖脂可能在猪咽上皮中作为猪链球菌半乳糖结合菌株的受体发挥作用。
