Watanabe M, Nagamine T, Sakimura K, Takahashi Y, Kondo H
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
J Comp Neurol. 1993 Jan 15;327(3):350-8. doi: 10.1002/cne.903270304.
The gene expression for alpha and gamma subunits of enolase, a dimeric enzyme in the glycolytic pathway, was examined in the developing brain of rats by in situ hybridization. The expression for the gamma subunit of enolase was first detected in post-mitotic neurons settled in the mantle zone at E13, and it increased progressively until the adult stage. Expression signals for the alpha subunit were discerned in two discrete regions showing different developmental changes: the signals in the proliferative ventricular zone were intense at E13 and decreased and eventually disappeared around birth, whereas the signals in the mantle zone persisted until the adult stage. In the adult brain, mRNAs for the alpha and gamma subunits were expressed widely in neurons, resulting in almost similar temporal patterns in the brain except for the cerebellum. Expression levels of the alpha subunit in adult glial cells were below the detection threshold of the in situ hybridization analysis. These findings suggest that both alpha and gamma enolase subunits participate in energy production in neurons of the mature brain and that marked changes in the subunit composition of enolase occur according to both neuron type and maturation.
通过原位杂交技术，在大鼠发育中的大脑中检测了烯醇化酶（糖酵解途径中的一种二聚体酶）的α和γ亚基的基因表达。烯醇化酶γ亚基的表达首先在E13定居于套层区的有丝分裂后神经元中被检测到，并逐渐增加直至成年期。α亚基的表达信号在两个显示不同发育变化的离散区域中被识别：增殖性脑室区的信号在E13时强烈，在出生前后减少并最终消失，而套层区的信号持续到成年期。在成体大脑中，α和γ亚基的mRNA在神经元中广泛表达，除小脑外，在大脑中产生几乎相似的时间模式。成体神经胶质细胞中α亚基的表达水平低于原位杂交分析的检测阈值。这些发现表明，α和γ烯醇化酶亚基都参与成熟大脑神经元的能量产生，并且烯醇化酶亚基组成根据神经元类型和成熟度发生显著变化。
