Watanabe E, Akagi H
Department of Anesthesiology and Reanimatology, Gunma University School of Medicine, Japan.
Neurosci Res. 1995 Nov;23(4):377-82. doi: 10.1016/0168-0102(95)00972-V.
The developmental changes in the expression of mRNAs encoding the alpha 1 and alpha 2 subunits of inhibitory glycine receptors in the spinal cord of fetal and postnatal rats were examined by in situ hybridization. During embryonic periods (E11-18), the mantle zone was scarce in the alpha 1 mRNA, but the germinal zone (matrix layer) at E11-14 expressed higher levels of the message. At postnatal day 0 (P0), the alpha 1 signals became manifested throughout the gray matter of the spinal cord. The intensities of the signals were increased to reach a maximal level at P21. By contrast, the spinal tissues at P0 exhibited the highest levels of alpha 2 mRNA, which decreased with the postnatal development. In P50 rats, the alpha 2 mRNA was barely expressed in the ventral horn, but a significant number of grains could still be detectable in a population of cells in the dorsal horn. During postnatal development from P0 to P10, the spinal tissues were rich in the alpha 1 and alpha 2 mRNAs, both of which were detected in the presumed motoneurons. The coexistence of the two subunits in single neurons might correlate with the modification of the glycine receptor function during the development of the spinal cord.
通过原位杂交技术，研究了胎鼠和新生大鼠脊髓中编码抑制性甘氨酸受体α1和α2亚基的mRNA表达的发育变化。在胚胎期（E11 - 18），α1 mRNA在套层中较少，但在E11 - 14的生发层（基质层）表达水平较高。出生后第0天（P0），α1信号在脊髓灰质中均有显现。信号强度在P21时增加到最高水平。相比之下，P0时脊髓组织中α2 mRNA水平最高，且随出生后发育而降低。在P50大鼠中，α2 mRNA在腹角几乎不表达，但在背角的一群细胞中仍可检测到大量颗粒。在从P0到P10的出生后发育过程中，脊髓组织中富含α1和α2 mRNA，二者在假定的运动神经元中均有检测到。单个神经元中这两个亚基的共存可能与脊髓发育过程中甘氨酸受体功能的改变有关。
