Lázár G, Maderdrut J L, Trasti S L, Liposits Z, Tóth P, Kozicz T, Merchenthaler I
Department of Anatomy, University Medical School, Pécs, Hungary.
J Comp Neurol. 1993 Jan 22;327(4):551-71. doi: 10.1002/cne.903270407.
The distribution of proneuropeptide Y-containing perikarya and nerve fibers in the brain of Rana esculenta and Xenopus laevis was determined with antisera directed toward neuropeptide Y and the carboxyl terminal flanking peptide. The distribution of proneuropeptide Y-like immunoreactivity was similar in both anurans. In the telencephalon, immunoreactive perikarya were found in the olfactory bulb, all subdivisions of the pallium, the septum, pars lateralis of the amygdala, the nucleus accumbens, and the anterior preoptic area. In the diencephalon, labelled perikarya were detected in the ventromedial, ventrolateral and central thalamic nuclei, the magnocellular preoptic nucleus, the suprachiasmatic nucleus, the posterior tuberculum, and the infundibulum. Amacrine-like cells were stained in the retina. In the pretectal area, posterior thalamic neurons showed intense, Golgi-like immunostaining. In the mesencephalon, immunoreactive cells were found in the reticular nucleus, the anteroventral tegmental nucleus, the optic tectum, the interpeduncular nucleus, and the torus semicircularis. In the rhombencephalon, labelled perikarya were detected in the secondary visceral nucleus, the central gray, the nucleus of the solitary tract, the dorsal column nuclei, and the spinal nucleus of the trigeminal nerve. Immunoreactive nerve fibers were observed in all areas of the brain that contained labelled perikarya. The densest accumulations were found in the accessory olfactory bulb, pars lateralis of the amygdala, the ventral habenula, the posterior pituitary, the optic tectum, the interpeduncular nucleus, and the saccular nucleus. The distribution of proneuropeptide Y-like immunoreactivity in the anuran brain showed many similarities to the distribution described for the amniote brain.
用针对神经肽Y和羧基末端侧翼肽的抗血清，确定了食用蛙和非洲爪蟾脑中含前神经肽Y的核周体和神经纤维的分布。两种无尾两栖类动物中前神经肽Y样免疫反应性的分布相似。在端脑，免疫反应性核周体见于嗅球、大脑皮质的所有亚区、隔区、杏仁核外侧部、伏隔核和视前区前部。在间脑，标记的核周体见于丘脑腹内侧核、腹外侧核和中央核、视前大细胞核、视交叉上核、后结节和漏斗。视网膜中的无长突细胞样细胞被染色。在顶盖前区，丘脑后部神经元呈现强烈的、类似高尔基染色的免疫染色。在中脑，免疫反应性细胞见于网状核、腹侧被盖前核、视顶盖、脚间核和半环形隆凸。在菱脑，标记的核周体见于次级内脏核、中央灰质、孤束核、薄束核和三叉神经脊束核。在脑内所有含有标记核周体的区域均观察到免疫反应性神经纤维。最密集的聚集见于副嗅球、杏仁核外侧部、腹侧缰核、垂体后叶、视顶盖、脚间核和球囊核。无尾两栖类动物脑中前神经肽Y样免疫反应性的分布与羊膜动物脑的分布有许多相似之处。
