Villalba R M, Martínez-Murillo R, Polak J M, Rodrigo J
Neuroanatomy Unit, Instituto Cajal, C.S.I.C., Madrid, Spain.
Cell Tissue Res. 1994 Jul;277(1):177-81. doi: 10.1007/BF00303094.
The present study provides light- and electron-microscopic immunocytochemical data on the presence of neurons that are immunoreactive to the C-terminal flanking peptide of neuropeptide Y, C-PON, in the neostriatum of the hedgehog (Erinaceus europaeus). Positive neurons have mostly fusiform or round perikarya from which two to four poorly branched processes arise. Immunostained fibers and puncta are also evenly distributed throughout the neostriatum. Ultrastructurally, each neuron exhibits a deeply invaginated nucleus surrounded by abundant cytoplasm with a well-developed rough endoplasmic reticulum and Golgi apparatus. Positive neurons receive symmetric and asymmetric synapses from unlabeled terminals. The results of this study can be correlated with previous findings, as the C-PON-positive neurons of the hedgehog resemble medium-sized neostriatal neurons that are known to be local circuit neurons exhibiting C-PON in the rat. Thus, a high degree of C-PON neuronal system phylogenetic conservation and function can be postulated for the neostriatum of mammals.
本研究提供了关于刺猬（欧洲刺猬）新纹状体中对神经肽Y的C末端侧翼肽C-PON具有免疫反应性的神经元存在情况的光镜和电镜免疫细胞化学数据。阳性神经元大多具有梭形或圆形的胞体，从胞体发出两到四个分支较少的突起。免疫染色的纤维和斑点也均匀分布于整个新纹状体。在超微结构上，每个神经元都有一个深陷的细胞核，周围是丰富的细胞质，有发达的粗面内质网和高尔基体。阳性神经元接受来自未标记终末的对称和不对称突触。本研究结果可与先前的发现相关联，因为刺猬的C-PON阳性神经元类似于大鼠中已知的作为局部回路神经元且表现出C-PON的中等大小新纹状体神经元。因此，可以推测哺乳动物新纹状体中C-PON神经元系统具有高度的系统发育保守性和功能。
