Cox G, Moseley P, Hunninghake G W
Department of Medicine, University of Iowa College of Medicine, Iowa City.
J Infect Dis. 1993 Mar;167(3):769-71. doi: 10.1093/infdis/167.3.769.
The expression of heat-shock protein (HSP) 70, a marker of cellular stress, was evaluated in neutrophils. In cells isolated by conventional method, expression of both the inducible HSP72 and the constitutive HSP73 forms of HSP70 was found. This was observed when cells were isolated at room temperature or at 4 degrees C. When autologous plasma was used during the isolation procedure and the entire procedure was done at 37 degrees C, there was no expression of HSP72. Omission of plasma from the isolation procedure done at 37 degrees C also resulted in increased expression of HSP72. These studies demonstrate that the expression of HSP70 in neutrophils is induced by isolation at low temperatures and in the absence of plasma. These observations may be important for studies of the regulation of HSP expression and for other studies that relate to neutrophil activation.
热休克蛋白（HSP）70是一种细胞应激标志物，对其在中性粒细胞中的表达进行了评估。在用传统方法分离的细胞中，发现了诱导型HSP72和组成型HSP73两种形式的HSP70的表达。在室温或4℃下分离细胞时观察到了这种情况。当在分离过程中使用自体血浆且整个过程在37℃下进行时，没有HSP72的表达。在37℃下进行的分离过程中省略血浆也导致HSP72的表达增加。这些研究表明，中性粒细胞中HSP70的表达是由低温分离和无血浆状态诱导的。这些观察结果对于HSP表达调控的研究以及其他与中性粒细胞活化相关的研究可能具有重要意义。
