Eid N S, Kravath R E, Lanks K W
J Exp Med. 1987 May 1;165(5):1448-52. doi: 10.1084/jem.165.5.1448.
Mature human neutrophils from peripheral blood are known to be capable of limited protein biosynthetic activity. We now show that these cells are inducible for heat-shock protein synthesis when placed in short-term culture. Synthesis of hsp70 and hsp85 as well as the response to various temperatures and the time course of induction were typical for mammalian cell systems. This heat-shock response was blocked by actinomycin D added before heat exposure. This demonstration that hsp genes can be activated by heat exposure of terminally differentiated neutrophils supports the hypothesis that gene activation can serve a physiological role in these cells and opens up the possibility that synthesis of other gene products is similarly inducible.
已知来自外周血的成熟人类中性粒细胞具有有限的蛋白质生物合成活性。我们现在表明，当置于短期培养中时，这些细胞可被诱导合成热休克蛋白。hsp70和hsp85的合成以及对各种温度的反应和诱导的时间进程对于哺乳动物细胞系统来说是典型的。这种热休克反应在热暴露前加入放线菌素D后被阻断。终末分化的中性粒细胞热暴露可激活hsp基因，这一证明支持了基因激活在这些细胞中可发挥生理作用的假说，并开启了其他基因产物的合成同样可被诱导的可能性。
