Saari H, Santavirta S, Nordström D, Paavolainen P, Konttinen Y T
Department of Anatomy, University Central Hospital, Helsinki, Finland.
J Rheumatol. 1993 Jan;20(1):87-90.
We studied the composition of the fluid in joint cavity of the hip joint in primary osteoarthritis (OA) from samples obtained at primary total hip replacement (THR) operations (N = 21) and in the THR pseudojoint, where the samples were obtained in revision operations for the common type of prosthesis loosening (N = 17). Protein concentrations differed little in both groups (31.8 +/- 1.9 mg/ml vs 34.4 +/- 1.9 mg/ml, p < 0.01), which suggests similar transcapillary flow in the degenerative hip joint and THR pseudojoint. Hyaluronate was found in OA joint fluid and in the fluid from the THR pseudojoint (2.21 +/- 0.23 mg/ml vs 0.43 +/- 0.04 mg/ml, p < 0.005) and high performance liquid chromatography (HPLC) analysis, calibrated using laser light scatter analyzed molecular weight standards, disclosed the low molecular weight nature of the OA synovial fluid compared to the THR pseudojoint fluid (1.12 +/- 0.84 x 10(6) vs 2.63 +/- 1.13 x 10(6) Da, NS). This suggests that THR arthroplasties cause an adaptive tissue response and formation of fibroblast-like B type lining cells able to synthesize and secrete hyaluronate.
我们从初次全髋关节置换（THR）手术中获取的样本（N = 21）研究了原发性骨关节炎（OA）患者髋关节腔液的成分，并在THR假关节中进行了研究，在翻修手术中针对常见类型的假体松动获取了样本（N = 17）。两组的蛋白质浓度差异不大（31.8±1.9 mg/ml对34.4±1.9 mg/ml，p < 0.01），这表明在退行性髋关节和THR假关节中跨毛细血管流动相似。在OA关节液和THR假关节液中均发现了透明质酸盐（2.21±0.23 mg/ml对0.43±0.04 mg/ml，p < 0.005），并且使用激光光散射分析的分子量标准进行校准的高效液相色谱（HPLC）分析显示，与THR假关节液相比，OA滑液具有低分子量特性（1.12±0.84 x 10(6)对2.63±1.13 x 10(6) Da，无显著性差异）。这表明THR关节成形术会引起适应性组织反应，并形成能够合成和分泌透明质酸盐的成纤维细胞样B型衬里细胞。
